recombinant human rh tgfβ Search Results


recombinant human (rh) tgfβ 5 ng/ml  (PeproTech)
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PeproTech recombinant human (rh) tgfβ 5 ng/ml
Inflammatory <t>cytokines</t> are upstream regulators of gene expression changes between neonatal and fetal stages and induce increased expression of brush border, absorption, and mucosal defense markers in vitro (A) Ingenuity pathway analysis showed inflammatory cytokines IFNγ, TNFα, IL1β, and TGFβ1 as upstream regulators of intestinal changes between late and early gestational age tissue and between neonatal versus fetal tissue. Activation Z score is presented by number and color. Orange color represents activation status of upstream regulator while blue signifies inhibition. (B–K) RT-qPCR analyses of maturation markers in proximal (red) and distal (blue) small intestine HFOs of (B and C) IAP , (D and E) LCT , (F and G) SIS , (H and I) FABP6 and (J and K) PIGR . n = 12 independent donors, data are plotted as paired values of individual cultures. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 as determined by non-parametric one-way ANOVA. See also <xref ref-type=Figures S2–S4 . " width="250" height="auto" />
Recombinant Human (Rh) Tgfβ 5 Ng/Ml, supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 1 article reviews
recombinant human (rh) tgfβ 5 ng/ml - by Bioz Stars, 2026-03
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Inflammatory cytokines are upstream regulators of gene expression changes between neonatal and fetal stages and induce increased expression of brush border, absorption, and mucosal defense markers in vitro (A) Ingenuity pathway analysis showed inflammatory cytokines IFNγ, TNFα, IL1β, and TGFβ1 as upstream regulators of intestinal changes between late and early gestational age tissue and between neonatal versus fetal tissue. Activation Z score is presented by number and color. Orange color represents activation status of upstream regulator while blue signifies inhibition. (B–K) RT-qPCR analyses of maturation markers in proximal (red) and distal (blue) small intestine HFOs of (B and C) IAP , (D and E) LCT , (F and G) SIS , (H and I) FABP6 and (J and K) PIGR . n = 12 independent donors, data are plotted as paired values of individual cultures. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 as determined by non-parametric one-way ANOVA. See also <xref ref-type=Figures S2–S4 . " width="100%" height="100%">

Journal: iScience

Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier

doi: 10.1016/j.isci.2024.109909

Figure Lengend Snippet: Inflammatory cytokines are upstream regulators of gene expression changes between neonatal and fetal stages and induce increased expression of brush border, absorption, and mucosal defense markers in vitro (A) Ingenuity pathway analysis showed inflammatory cytokines IFNγ, TNFα, IL1β, and TGFβ1 as upstream regulators of intestinal changes between late and early gestational age tissue and between neonatal versus fetal tissue. Activation Z score is presented by number and color. Orange color represents activation status of upstream regulator while blue signifies inhibition. (B–K) RT-qPCR analyses of maturation markers in proximal (red) and distal (blue) small intestine HFOs of (B and C) IAP , (D and E) LCT , (F and G) SIS , (H and I) FABP6 and (J and K) PIGR . n = 12 independent donors, data are plotted as paired values of individual cultures. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001 as determined by non-parametric one-way ANOVA. See also Figures S2–S4 .

Article Snippet: HFOs donors were stimulated for 72 h with the following cytokines: recombinant human (rh) IFNγ 0,5 ng/ml (R&D systems, cat#285-IF), rhTNFα 2ng/ml (Peprotech, cat#:300-01A), rhIL1β 5 ng/ml (Thermo Fisher Scientific, cat#PHC0814), rhTGFβ 5 ng/ml (Peprotech, cat#:100-21A).

Techniques: Gene Expression, Expressing, In Vitro, Activation Assay, Inhibition, Quantitative RT-PCR

IFNγ and cytokine mix stimulation increases IAP activity and FABP6 protein expression in HFOs (A and B) Alkaline phosphatase activity level (μg pNPP/min) in (A) proximal and (B) distal HFOs upon cytokines stimulation ( n = 9 independent donors). (C) Alkaline phosphatase (IAP) staining of embedded HFOs upon IFNγ, and cytokine mix stimulation compared to control. (D) FABP6 protein expression measured by western blot in distal HFOs. (E) FABP6 western blot quantification on n = 5 independent donors. ∗ p < 0.05, ∗∗ p < 0.01 as determined by non-parametric one-way ANOVA.

Journal: iScience

Article Title: Pro-inflammatory T cells-derived cytokines enhance the maturation of the human fetal intestinal epithelial barrier

doi: 10.1016/j.isci.2024.109909

Figure Lengend Snippet: IFNγ and cytokine mix stimulation increases IAP activity and FABP6 protein expression in HFOs (A and B) Alkaline phosphatase activity level (μg pNPP/min) in (A) proximal and (B) distal HFOs upon cytokines stimulation ( n = 9 independent donors). (C) Alkaline phosphatase (IAP) staining of embedded HFOs upon IFNγ, and cytokine mix stimulation compared to control. (D) FABP6 protein expression measured by western blot in distal HFOs. (E) FABP6 western blot quantification on n = 5 independent donors. ∗ p < 0.05, ∗∗ p < 0.01 as determined by non-parametric one-way ANOVA.

Article Snippet: HFOs donors were stimulated for 72 h with the following cytokines: recombinant human (rh) IFNγ 0,5 ng/ml (R&D systems, cat#285-IF), rhTNFα 2ng/ml (Peprotech, cat#:300-01A), rhIL1β 5 ng/ml (Thermo Fisher Scientific, cat#PHC0814), rhTGFβ 5 ng/ml (Peprotech, cat#:100-21A).

Techniques: Activity Assay, Expressing, Staining, Control, Western Blot